Development of an anti-TFF3 functionalized surface to capture of Barrett’s oesophagus cells

The Idea

We have successfully developed a non-endoscopic diagnostic test involving a cell-collection device that, coupled with the biomarker trefoil factor 3 (TFF-3), diagnoses Barrett’s oesophagus, the only known precursor of oesophageal adenocarcinoma. We propose to fabricate an anti-TFF3 functionalized surface to capture of Barrett’s oesophagus cells. This prototype will provide the backbone for the development of microfluidic device for capture of Barrett’s oesophagus cells and further characterization rom Cytopsponge samples. 

Figure 1. Cytosponge pulled from the stomach and collecting cells from the oesophageal lining.

The Team

 Gianmarco Contino (Rebecca Fitzgerald Lab, MRC-CU, Cambridge University), designed the project and provide the biological expertize and reagents to design and optimize the functionalized surface. 

Miranda Robbins, (Department of Chemical Engineering and Biotechnology, University of Cambridge) manufactures the functionalized surface and collaborate to the testing.

---

Project Outcomes

Project Report

Project report and documentation on Github

Project Proposal

Original proposal and application

Project Resources

Hardware Schematics

Bill of Materials

Software Code